I am using a retroviral system to transduce primary cells. We already have a working system in the lab using an MMLV-based vector together with the platE packaging cell line from Cell biolabs.
cell, and how the silencing of retroviral DNAs is achieved is not yet well characterized. Results: In the current study, we investigated the transcriptional silencing dynamics in stem cells by independently monitoring the expression of two Moloney murine leukemia virus (MMLV) retroviral vectors newly introduced into embryonic carcinoma (EC) cells.
leukemia virus (MMLV). Here, we have shown that immunovirosomes carrying monoclonal antibodies against endothe-lial markers can mmlv retroviral vector target activated vascular endothelium. The resulting gene transfer efficiency is enhanced if endocytic recep-tors are chosen. Importantly, our results demonstrate that changing viral tropism
Studies comparing the lentivirus HIV and the retrovirus MMLV indicate that the viral integrase plays a mmlv retroviral vector role in shaping integration site preferences. A major cellular determinant is LEDGF/p75, a lentiviral tethering protein that recruits the pre-integration complex to transcriptional units and facilitates integration.
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