MMLV cis- and trans- acting packaging elements. (A) The MMLV genome. The three MMLV protein-coding sequences are marked, as are the splice donor (SD) and splice acceptor (SA) sites.
M-MLV Reverse Transcriptase Buffer is supplied as 5X Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2] with 500 l 0.1 M DTT. It is included with M-MLV, SuperScript, SuperScript II, and SuperScript III reverse transcriptases.
Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase is an RNA-directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. (1-4). M-MuLV Reverse Transcriptase lacks 3´ → 5´ exonuclease activity. Product Source
Sep 05, 2018 · This novel procedure uses the template-switching activity of Moloney murine leukemia virus (MMLV) reverse transcriptase to synthesize and anchor first-strand cDNA in one step. Following reverse transcription, three cycles of PCR are performed using a modified oligo(dT) primer and an anchor primer to enrich the cDNA population for fulllength ...
Retrovirus (e.g. MMLV) can introduce genetic material into the genome of the host cell, making it great for long-term stable expression. MMLV-based retroviruses, however, cannot infect non-dividing cells. Retroviral titer and mmlv genome transduction efficiency is typically lower than that of other viral vectors.
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